HPLC-DAD

HPLC-DAD ( High Performance Liquid Chromatography Diode Array Detector)

Carrier solvent: liquid, usually Methanol-Water Mixture
Stationary phase: Silicon related compound
DAD: UV ray

Analyte after separation, will be detected by DAD, which uses UV ray, DAD will detect the optical density of compound. A UV spectra of the compound will be produce, the spectra will , match with the reference spectra to determine the identity of compound. HPLC is therefore suitable for compound like
Allergens
Toxin
Antibiotics
Growth promoters (hormones)

Aflatoxin and Orchratoxin

The above toxin has a special reaction UV ray. In the presence of UV ray, toxins will fluorescence at different wavelength. E.g. Orchratoxin at 330nm. Therefore HPLC is very suitable for detection of the above captioned toxin.